Effect of silver nanoparticles on Escherichia coli isolates from urinary tract infections resistant to several antibiotics

Urinary tract infection is one of the most common and common bacterial infections, accounting for a significant proportion of hospital admissions (about 30-40%). Silver nanoparticles work by releasing silver ions against various bacteria. The fact that bacteria are not resistant to nanoparticles is very important and therefore will affect a wide range of bacteria.

In this study, 50 specimens of positive cultures with urinary tract infection referred to Imam Reza Hospital Laboratory in Bojnourd were studied. Resistance and susceptibility of the isolates were determined by disk diffusion method. In this study, antibacterial effects of silver nanoparticles were investigated by microdilution method using aqueous extract of Ganoderma leucidum. Vegetative electron microscopy was used to measure the size and shape of silver nanoparticles. In addition, infrared spectroscopy analysis was performed to investigate possible organic compounds involved in the synthesis of nanoparticles.

The highest antibiotic resistance was related to ampicillin (84%). The resulting nanoparticles were 20 to 45 nm in size.

The produced nanoparticles have antimicrobial activity and can be a good alternative in the treatment of antibiotic resistant infectious diseases.