Study of B4GALNT2 (FecL) gene mutation by using High Resolution Melting (HRM) technique in Zandi sheep

Message:
Article Type:
Research/Original Article (دارای رتبه معتبر)
Abstract:

Litter size is one of the most important reproductive criteria in animals. Hence, the effect of this trait on the productivity of the flock has been addressed by its genetic enhancement through molecular technologies. Previous studies have shown that the major genes including GDF9, BMP15, ALK6, and B4GALNT2 (FecL) are associated with litter size. The effect of FecL mutation in homozygous states is additive and results in the release of three ovum for two copies of this gene. The aim of this study was to investigate polymorphism DLX3: c. * 803A> G in B4GALNT2 (FecL) gene on chromosome 11, using High Resolution Melting technique (HRM), and it is association with litter size in Zandi sheep. HRM analysis is a method that reduces the amount of fluorescence during the process of thermal melting of the DNA through color exhaust. The results of HRM were consistent with the sequencing results. No mutations were observed in the nucleotide sequence of the target studied in the Zandi sheep. Therefore, B4GALNT2 gene is monomorphic for this site length in 232bp fragment of this gene. We discovered many deletions, but there was no change in the resulting amino acid. Therefore, that did not result in structural alteration of the protein. In addition, two new mutations included A> G mutation at position 2242 in sample 7 and mutation C> G at position 2241 in sample 77 were detected. It seems necessary to investigate mutations in other major genes for this breed

Language:
Persian
Published:
Journal of Genetics, Volume:17 Issue: 1, 2022
Pages:
87 to 90
magiran.com/p2423167  
دانلود و مطالعه متن این مقاله با یکی از روشهای زیر امکان پذیر است:
اشتراک شخصی
با عضویت و پرداخت آنلاین حق اشتراک یک‌ساله به مبلغ 1,390,000ريال می‌توانید 70 عنوان مطلب دانلود کنید!
اشتراک سازمانی
به کتابخانه دانشگاه یا محل کار خود پیشنهاد کنید تا اشتراک سازمانی این پایگاه را برای دسترسی نامحدود همه کاربران به متن مطالب تهیه نمایند!
توجه!
  • حق عضویت دریافتی صرف حمایت از نشریات عضو و نگهداری، تکمیل و توسعه مگیران می‌شود.
  • پرداخت حق اشتراک و دانلود مقالات اجازه بازنشر آن در سایر رسانه‌های چاپی و دیجیتال را به کاربر نمی‌دهد.
In order to view content subscription is required

Personal subscription
Subscribe magiran.com for 70 € euros via PayPal and download 70 articles during a year.
Organization subscription
Please contact us to subscribe your university or library for unlimited access!