Production of specific monoclonal antibody to Xanthomonas translucens pathovars, the causal agents of bacterial leaf streak of cereals

In order to be used in studies related to the production of a kit for detection of the causal agent of bacterial leaf streak of cereals, in contaminated wheat and barley seeds, a hybridoma cell line (named L-B7) secreting monoclonal antibody to Xanthomonas translucens pv. translucens by fusing SP2/0 myeloma cells with splenocytes from immunized BALB/c mice with one strain of this bacteria (UPB684 isolated from barley in Iran), were produced. The monoclonal antibody produced, reacted positively with X. c. pv. undulosa (15 strains) and pv. translucens (15 strains). Strains from other pathovars (X. c. pv. graminis and pv. poae) gave a negative reaction. Antibody potency was assessed by testing in ELISA. Whole cells were used both as immunogen and as antigen in ELISA tests. This antibody could be used to differentiate Xanthomonas translucens pathovars, causing bacterial leaf streak of cereals, from other pathovars, as well as in the process of producing a detection kit.