The inhibitory effect of Thymus vulgaris and Carum copticum essential oil on the growth of Rhizoctonia solani, the causal agent of potato stem canker in vitro and greenhouse conditions
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Article Type:
Research/Original Article (بدون رتبه معتبر)
Abstract:

Potato is one of the most important food and economic crops in all over the world, Rhizoctonia solani, the causal agent of stem canker causes high loss of the product quality and quantity annually. In this study, the effects of two essential oils of Thyme and Carum, were investigated on the colony growth rate and inhibitory growth in concentrations of 100, 200, 300, 400 and 500 ppm on PDA medium. In vitro tests, essential oils of thyme and Carum in two concentrations of 0.5 and 0.75, the combination of thyme 0.25+ Carum 0.25 and Rovral-Ts fungicide at 1.5 at 1000 were evaluated in a completely randomized block design in four replications. The tubers substratum cultivated in soil contaminated by R. solani that planted in pots were inoculated by essential oils and fungicides. The disease severity data were recorded and scored six weeks after inoculation using Das scale. The results demonstrated that fungal growth rate under treatment by Thyme at 500 ppm (21.63 mm /day) and Carum (17.7 mm/day) and thyme + Carum (67.85) essences were more than 50 percent growth inhibitory. The results of disease severity index in greenhouse revealed that Thyme and Carum essences and Rovral-Ts fungicide respectively by 28.1, 35.92 and 17.17 percent were successful in disease control as well as the mean of fresh and dry weight of shoot area under Thyme treatment increased relatively.

Language:
Persian
Published:
Journal of Applied Plant Protection, Volume:6 Issue: 2, 2017
Pages:
97 to 107
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