Histological and Anatomical Study of Tympanic bulla in Adult River Buffalo

Buffalo has a special role in the economy of more than forty countries and theirs numbers and distributions is growing every year. Tympanic bulla content middle ear. There is no anatomical and histological research performed on this organ in buffalo. For this study 16 adult male and female buffalo's head were provided from slaughter house and their tympanic bulla were studied comparative anatomical and histologically, then tissue samples were obtained and paraffin sections were prepared for using of staining method Haematoxylin and eosin. Anatomical results showed tympanic bulla in male and female buffalo’s was similar large, extended and elliptical . Bone wall was thin and fragile. Muscular process in tympanic bulla was unique and non- sharp. Ossicles and tympanic membrane tympanic were observed in depth of tympanic bulla. Histological results showed bones of tympanic bulla in male and female buffalo’s was trabecular bone. Epithelium of tympanic bulla was simple squamous epithelium in most parts. In the terminal part of tympanic bulla ( near to middle ear) epithelium was pseudostratified ciliated columnar. The average measurement of parameters showed the size of tympanic bulla ossicles in female is larger than male, and right side also was larger than left side. Statistical analysis Tuckey showed these differences were not significant (p≤0/05).