Comparison of physicochemical properties and fatty acid profile of seed oil from Citrus aurantium L. extraction in different extraction methods

Citrus aurantium L. seed is a by-product of fruit juice industries. In this research, the main fatty acids and phytosterols in C. aurantium seed oil were evaluated with the aim of nutritional evaluation and its biological properties. The C. aurantium oil extracted by two methods of cold pressing and using hexane solvent.The C. aurantium seed fatty acids composition was analyzed by GC-MS, 1HNMR, and 13CNMR. In addition, the effects of ultrasonic waves and air pressure on the C. aurantium pulp obtained from cold press extraction by n-hexane was studied. The findings showed that the highest oil yields were obtained using ultrasound-assisted extraction (25%) compared to other methods. The five fatty acids including 9-oleic (18.07%), Linoleic (36.69%), palmitic (21.90%), stearic acid (6.6%) and 10-oleic acids (1.1%) were the most phytosterols in C. aurantium seed oil. According to the obtained results, C. aurantium oil can be considered as a new food source.