The physical and chemical data of the pure substance can be analysed to determine the structure. In the current era of structural elucidation, NMR spectroscopy and mass spectroscopy, especially the various types of 2D NMR, are used extensively, while infrared (IR) and ultraviolet spectroscopy (UV) are main tool to recognize the functional groups in the structure. The correlation of key chemicals in chromatograph peaks with individual mass spectra is commonly accomplished using HPLC mass spectrometry (LCMS). For most chemicals studied, LCMS ionisation procedures are chemically mild, and strong molecular ion peaks are detected. MS (mass spectrometry) is a technique for determining a substance's molecular mass, formula, and fragmentation pattern. The most widely deployed techniques in mass spectrometry are electrospray ionisation mass spectrometry, chemical ionization mass spectrometry, chemical ionisation mass spectrometry, and fast atom bombardment mass spectrometry. Our paper discusses in brief how mass spectrometry can be used to study and analyze natural products in a scientific manner. Mass spectrometry, which has been used to study complex biological systems for more than a century, is currently at a critical crossroads. We'll be looking at how the mass spectrometry business is expected to change over the next decade, emphasising the natural products industry's increasing techniques and technology. This research aims to provide a fast summary of how mass spectrometry is used in many disciplines. This review study relies on three search engines, Pub Med, Science Direct, Google Scholar, and printed material from the library, to do the best literature research possible. We highlight growing mass spectrometric methodologies and technologies employed by the natural product field in this viewpoint piece and provide a glimpse into the future directions in which the mass spectrometry field will migrate over the next decade.
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