Association between HOTAIR rs1899663 G>T Gene Polymorphism and Thyroid Cancer Susceptibility

Studies show that single nucleotide polymorphisms of the HOTAIR gene are associated with a variety of cancers, including colorectal, breast, and thyroid cancers. The product of the HOTAIR gene is a long non-coding RNA that is involved in regulating gene expression, cell cycle and apoptosis and is considered an oncogene. The present study was conducted to investigate the association between HOTAIR rs1899663 G>T gene polymorphism and thyroid cancer susceptibility in northwestern Iran.

In this case-control study, peripheral blood was obtained from 90 patients with thyroid cancer referred to Tabriz hospitals as well as 198 healthy individuals. After DNA extraction by saturated salt and proteinase K method, single nucleotide polymorphism of HOTAIR rs1899663 gene was examined by tetra-primers ARMS PCR. Then, allelic and genotypic frequencies of control and case groups were calculated and compared.

In this study, the genotypic frequencies of GG, GT and TT in the case group were 23.3%, 49% and 27.7%, and in the control group were 20.2%, 41% and 38.8%, respectively. Allele frequencies of G and T were 48% and 52.2% in the case group and 41% and 59% in the control group, respectively. Allelic and genotypic comparisons between case and control groups showed no significant relationship.

The results of the study showed that HOTAIR rs1899663 gene polymorphism is not associated with any of the clinicopathological features of thyroid cancer.