Pseudomonas fluorescens bacteria are the most important rhizosphere bacteria that have a positive effect on vegetative and biochemical traits of the plant. In this study, genetic diversity of 22 Pseudomonas fluorescens strains and their effect on defense enzymes and growth factors of wheat plant were performed in three stages of greenhouse, molecular and biochemical evaluation. In molecular evaluation, 12 RAPD primers were used to evaluate the genetic diversity of strains. Biochemical evaluation was performed in 4 stages of 0, 3, 6 and 9 days after inoculation on wheat to measure the defense enzymes peroxidase and phenylalanine ammonialyase. VUPF5 strain on wheat increased shoot and biological weight. RAPD-PCR separated strains with similar geographic location and similar host. Greenhouse, molecular, and biochemical groupings identified almost the top strains. Strains 680, 738, 50, 354, 49, 58 and VUPF5, which had a positive effect on molecular evaluation in one group, were shared with the superior group of enzymes. T26-2 and F-68 in OP-I15 and OP-H8 primers amplified 1300 bp and 1200 bp bands, respectively witch these bands can evaluated after be further studies as candidate bands in the expression of these enzymes in wheat.
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