Isolation and identification of L-asparaginase producing bacteria from soil and effluent of slaughterhouse in Isfahan

Article Type:
Research/Original Article (دارای رتبه معتبر)
L-asparaginase is one of the most important treatments for acute lymphoblastic leukemia. The aim of this study was isolation and molecularly identification for L-asparaginase producer bacteria. In this study, samples were taken from the effluent and soil of a slaughterhouse in Isfahan. M9 culture medium was used to screen the L-asparaginase producer bacteria. After screening, qualitative measurement of L-asparaginase was performed based on the color change in the media. The L-asparaginase producer bacteria were identified by biochemical and molecular methods. 114 colonies were isolated from the samples. 5 colonies were able to produce L-asparaginase. The halo diameter of L-asparaginase production in bacteria numbers SS18, SS19, SE64, SE112 and SE114 were 21, 22, 30, 33 and 55 mm, respectively. 16SrRNA sequences showed that strain SS18 was 99% likely to belong to Stenotrophomonas maltophilia, strain SS19 was 99% likely to belong to Chryseobacterium indologenes, strain SE64 was 99% likely to belong to the genus Chryseobacterium, strain SE112 was 99% likely to belongs to Bacillus safensis species and strain number SE114 probably 100% belongs to Bacillus velezensis species.  Since, L-asparaginase from different bacteria has shown different anti-cancer effects, finding the L-asparaginase producer microorganisms is one of the ways that lead us to have an enzyme with ideal medical aspects. One of the best environments for finding these bacteria is slaughterhouse soil and effluent, which are high in protein and can therefore be a potential source of bacteria that produce L-asparaginase.
International Journal of Molecular and Clinical Microbiology, Volume:12 Issue: 2, Summer and Autumn 2022
1704 to 1712  
دانلود و مطالعه متن این مقاله با یکی از روشهای زیر امکان پذیر است:
اشتراک شخصی
با عضویت و پرداخت آنلاین حق اشتراک یک‌ساله به مبلغ 1,390,000ريال می‌توانید 70 عنوان مطلب دانلود کنید!
اشتراک سازمانی
به کتابخانه دانشگاه یا محل کار خود پیشنهاد کنید تا اشتراک سازمانی این پایگاه را برای دسترسی نامحدود همه کاربران به متن مطالب تهیه نمایند!
  • حق عضویت دریافتی صرف حمایت از نشریات عضو و نگهداری، تکمیل و توسعه مگیران می‌شود.
  • پرداخت حق اشتراک و دانلود مقالات اجازه بازنشر آن در سایر رسانه‌های چاپی و دیجیتال را به کاربر نمی‌دهد.
In order to view content subscription is required

Personal subscription
Subscribe for 70 € euros via PayPal and download 70 articles during a year.
Organization subscription
Please contact us to subscribe your university or library for unlimited access!