In order to investigate the effects of drought stress on grain yield, biological yield, yield components and harvest barley index of Gohar cultivar, an experiment was conducted as a randomized complete block design in the experimental farm of Shushtar Azad University. For this purpose, in field conditions, drought stress treatment at five levels was applied as irrigation interruption in the stages of tillering, stem emergence, flowering, seed filling and no irrigation interruption (control treatment). The amount of water consumed was measured by a volume meter. The results showed that there was a significant difference between drought stress treatments in all studied traits except harvest index. Stress-free treatment produced the highest grain yield by producing the highest biological yield, number of spikes per unit area and number of grains per high spike and suitable 1000-grain weight. In terminal stress conditions or cessation of irrigation in the grain filling stage after non-stress conditions had a higher yield than other stages. The study of correlation coefficients showed that among the yield components, the number of spikes per unit area had the highest correlation (0.63) with grain yield. In this experiment, the lowest grain yield was obtained in the cut-off treatment at the flowering stage, which indicates the sensitivity of this stage of plant growth to dehydration stress