LOADING OF PECAN SMOKE EXTRACT BY CHITOSAN NANOPARTICLES AND EVALUATION OF ITS TOXICITY AND INDUCTION OF APOPTOSIS ON CANCER CELLS A2780

Nanoparticles are effective in cancer cells and in the treatment of diseases due to their ability to induce toxicity and induce apoptosis and antibacterial properties. Pecan seeds are rich in carbohydrates, lipids, proteins, mineral salts, alkaloids, and amino acids; among its important alkaloids are harmaline, harmine, harmalol and vazisine can be mentioned, which have many uses in industry and medicine. The aim of this research is to investigate the effects of pecan smoke loaded with nano-chitosan on cytotoxicity, apoptosis induction and its antibacterial effects.

In this experimental study which was conducted in vitro, MTT test was used to investigate the toxicity effect of chitosan nanoparticles loaded with pecan smoke on ovarian cancer cell line A2780 and HFF normal fibroblast cell. Also, to investigate the induction of apoptosis, the method of determining the expression of the caspase 3 gene was used, and to investigate the antibacterial properties, the disk diffusion method was used on the gram-positive bacteria Staphylococcus aureus and Micrococcus luteus and the gram-negative bacteria Escherichia coli and Pseudomonas aeruginosa. Statistical analysis was performed by SPSS software and one-way analysis of variance. P<0.05 was considered as significant.

The result of the MTT test showed the value of IC50 for ovarian cancer cells in 48 hours after treatment as 17.155 μg/ml; while at the same concentration for the normal cell line, the survival rate was 98%. Also, the induction of apoptosis of A2780 cancer cells was observed based on a significant increase in the expression of caspase 3 gene. In addition, these nanoparticles had an inhibitory effect on the growth of Staphylococcus aureus and Micrococcus luteus bacteria.

Chitosan nanoparticles synthesized by Peganum harmalla smoke had anti-cancer, apoptotic, and antibacterial activity.