The Effect of Tamoxifen and Black Cohosh (Actaea racemosa) On The Changes In The Levels Of Sex Steroid Hormones And Oocytes Maturation Of Adult Female Gourami fish (Trichogaster trichopterus) from family (Osphronemidae)

One of the most common cancers in the menopausal age is breast cancer. To control menopause symptoms in women with breast cancer, given that hormone therapy is not recommended, alternative treatments such as phytoestrogen therapy can be considered. Black cohosh is a phytoestrogen plant that is used to control menopause symptoms. The aim of this study was to compare the effect of tamoxifen with black cohosh plant on ovarian tissue ultrastructure and measurement of sex hormones in adult female three-spot gourami fish.

For this purpose, the number of 120 pieces of three-spot gourami adult female fish with an average weight of 5±1 grams in 8 treatments with control groups including control 1 (intact) and control 2 (solvent, Ethanol 60 %) and treatments receiving doses of 10, 50, 100 mg/kg of tamoxifen drug and 10, 50, 100 mg/kg of black cohosh hydroalcoholic extract were divided. All the injections were done in 10 times and every other day for twenty days and in the amount of 0.02 ml between the dorsal fin muscle and the lateral line. Then, after anesthetizing the fish, the ovary was examined by light and electron microscope. For measuring the levels of steroid hormones, the fish tissue was homogenized by homogenizer.

Black cohosh extract decreased the level of 17-beta estradiol and testosterone hormones. Tamoxifen decreased the level of 17-beta estradiol, testosterone and progesterone hormones. Examining the light and electron microscope images showed that both tamoxifen and black cohosh had an inhibitory effect on oocyet maturation.

Conclusion:

 The results of this study showed that black cohosh plant extract and tamoxifen reduce oocyte maturation.