The effect of Polycaprolactone scaffold with Myrtus communis and silver nanoparticles on wound healing and antimicrobial properties on burn wound infection induced by Methicillin-resistant Staphylococcus aureus in rats

Regarding to the important role of Staphylococcus aureus in burn wound infection, the aim of the present study were to investigation of the effect of Polycaprolactone (PCL) scaffold with Myrtus communis extract on the healing of burn wound infections induced by Methicillin-resistant Staphylococcus aureus in rats.

In this experimental study, 42 male Wistar rats were divided into 7 equal groups. Second-degree burns developed on the backs of all animals after anesthesia by injection of a mixture of ketamine and xylazine, and then MRSA was inoculated uniformly on the wounds of all mice. Burn wounds were treated daily with Polycaprolactone (PCL) scaffold, PCL- myrtus 1%, PCL- myrtus 0.5%, PCL-silver sulfadiazine and PCL-Myrtus-silver sulfadiazine ointments. We investigated the antimicrobial properties, wound healing percentage, wound area, malondialdehyde and nitric oxide concentrations of rats. Data analysis was performed using GRAPH PAD software and One-way Anova and Tukey tests

Histopathological observations showed that PCL- myrtus group had wound healing properties in comparison with control group (p<0.05). PCL- myrtus group had reduced bacterial growth compared to PCL group and positive control group. Also biochemical tests showed a decrease in malondialdehyde and nitric oxide in rats treated with the PCL-Myrtus ointment compared to the other groups.

According to the results PCL containing myrtus ointment have an important role on accelerate the healing process, reducing time for wound healing and prevention of infection caused by MRSA.