Investigation the Effects of Curcumin Pretreatment of Mesenchymal Stem Cells Conditioned Medium on Heart Fibrosis Followed Induction of Myocardial Infarction

The aim of the present study was evaluating the protective effect of curcumin pretreatment of bone marrow mesenchymal stem cells conditioned medium on heart fibrosis in rat.

Eighteen male mice were randomly divided into three groups of six; Healthy control group: (normal saline), Patient control group: isoproterenol (ISO), Treatment group: ISO + condition medium (CM). Blood samples were then collected. Then the femur and tibia bones were separated and used for stem cell culture. Cell secretions extracted from bone marrow cells were injected through the tail vein. In order to perform the test, the obtained heart samples were fixed in formalin 10% and histologically evaluated. Also, serum levels of LDH (lactate dehydrogenase) and CPK (creatine phosphokinase) were measured.

Subcutaneous injection of ISO led to the induction of (myocardial infarction: MI) with increase in serum CPK and LDH compared to the normal control group (P < 0.05). Intravenous injection of conditioned medium resulted in a significant decrease in serum CPK and LDH levels compared with the MI group (P < 0.05). The mean severity of fibrosis in the CM group was significantly decreased compared to the MI group, but the inflammation and edema of the heart in both groups were significantly different with normal group.

In the present study, single-dose injection of the CM of mesenchymal stem cells treated with curcumin can attenuate adverse effects of ISO and significantly reduced the cardiac fibrosis.