Frequency of Tetracycline Resistance in Enterococcus Isolated from food

Enterococcus as a part of the normal flora of human gastrointestinal tracts can cause infections in foods. Aim of this study was to determine tetracycline resistance in Enterococcus isolated from food. In this laboratory study, one hundred samples of food from different parts of Qom were collected. After determining the type, they were tested for their resistance against tetracycline genes by specific primer PCR. Out of 53 isolates, 45% isolates belonged to Enterococcus faecium 55% isolates were Enterococcus faecalis. Size band of this genes were tetS 647bp, tetO 442 bp, tetM 373 bp, tetL 306bp, ermb 311 bp. Among this samples tetO (12 %), tetS (6 %), tetM (17 %), tetL( 5%) and ermB (7%)isolate. Considering the results, increasing antibiotic resistant strains, especially tetracycline among Enterococcus, is a serious threat to the general public, Molecular method could help rapid diagnosis this resistance genes and help to treatment.