Prevalence and Characteristics of Yersinia Enterocolitica and Yersinia Pseudotuberculosis from Raw Milk Supplied in Tehran
Article Type:
Research/Original Article (دارای رتبه معتبر)

Foodborne diseases are caused by eating contaminated food. Yersinia species are among the bacteria involved in food contamination, such as meat, poultry, vegetables, and milk. This study aims to investigate the prevalence and characteristics of Yersinia enterocolitica and Yersinia pseudotuberculosis from raw milk in Tehran.


In this study, 360 samples of raw milk were collected from farms around Tehran and examined according to the FDA method. Then, 25 ml of milk samples were added to 225 ml of Peptone Sorbitol Bile Broth and kept for 10 days at 4 °C for enrichment. After 10 days, 0.5 ml of the sample was added to 0.5 ml of 0.72% KOH solution and 0.54% NaCl. After 30 sec, it was cultured in the selected Cefsulodin-Irgasan-Novobiocin agar (CINagar) medium to remove the normal flora. The plates were stored for 48 h at 28 °C. The suspected Bull's eye colonies were purified and phenotyping tests were carried out on the selected colony. The 20 E API kits were used for confirming identification.


From 360 raw milk samples, 4 Yersinia isolates (1.1%) including one Y. pseudotuberculosis (0.27%) and three Y. enterocolitica (0.83%) were isolated. In addition to Yersinia, other bacteria such as Klebsiella, Serratia, Citrobacter, and Providencia were isolated from milk samples.


The findings showed that clean tap water and healthy cattle in livestock can be effective in preventing Yersinia contamination. Lack of personal and environmental hygiene could cause food contamination by Yersinia and other intestinal bacteria leading to gastrointestinal infections.

Journal of Nutrition and Food Security, Volume:8 Issue: 1, Feb 2023
114 to 121  
دانلود و مطالعه متن این مقاله با یکی از روشهای زیر امکان پذیر است:
اشتراک شخصی
با عضویت و پرداخت آنلاین حق اشتراک یک‌ساله به مبلغ 750,000ريال می‌توانید 50 عنوان مطلب دانلود کنید!
اشتراک سازمانی
به کتابخانه دانشگاه یا محل کار خود پیشنهاد کنید تا اشتراک سازمانی این پایگاه را برای دسترسی نامحدود همه کاربران به متن مطالب تهیه نمایند!
  • حق عضویت دریافتی صرف حمایت از نشریات عضو و نگهداری، تکمیل و توسعه مگیران می‌شود.
  • پرداخت حق اشتراک و دانلود مقالات اجازه بازنشر آن در سایر رسانه‌های چاپی و دیجیتال را به کاربر نمی‌دهد.
In order to view content subscription is required

Personal subscription
Subscribe for 50 € euros via PayPal and download 50 articles during a year.
Organization subscription
Please contact us to subscribe your university or library for unlimited access!