The liver is exposed to poisoning caused by various chemical compounds. Considering the antioxidant effects found in melon seeds and the role of NF-κB and MPO in the occurrence of inflammation caused by oxidative stress, in the present study the effect of hydroethanolic extract of C. melo seeds on the transcriptional level of NF-κB and MPO genes in ethylene glycol hepatotoxicity induced rats was evaluated.
The number of 30 rats was randomly divided into 5 groups including healthy groups, poisoned control (0.75% ethylene glycol), and experimental (0.75% ethylene glycol and concentrations of 150, 300, and 500 mg/kg body weight of the extract) and were treated for 38 days. Then, the liver tissue of the rats was removed and transcriptional levels of NF-κB and MPO genes were evaluated by real-time PCR method.
The transcriptional level of the NF-κB and MPO in the liver of the poisoned control group rats has a significant increase compared to the healthy group. Treatment with hydroethanolic extract of C. melo seeds in concentrations of 300 and 600 mg/kg of body weight causes a significant decrease in the transcriptional level of the NF-κB gene in the experimental groups. The treatment of hydroethanolic extract of C. melo seeds in concentrations of 150, 300, and 600 mg/kg of body weight causes a significant decrease in the transcription of the MPO gene in the experimental groups compared to the poisoned control group.
The decrease in MPO and NF-κB indicates the therapeutic effect of melon seed extract on liver damage.
C. melo seed , liver toxicity , MPO , NF-κB
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