Evaluation of the effect of hydroethanolic extract of Cucumis melo seeds on transcriptional level of NF-κB and myeloperoxidase encoding genes in hepatocyte of ethylene glycol induced toxicity in male rats
The liver is exposed to poisoning caused by various chemical compounds. Considering the antioxidant effects found in melon seeds and the role of NF-κB and MPO in the occurrence of inflammation caused by oxidative stress, in the present study the effect of hydroethanolic extract of C. melo seeds on the transcriptional level of NF-κB and MPO genes in ethylene glycol hepatotoxicity induced rats was evaluated.
The number of 30 rats was randomly divided into 5 groups including healthy groups, poisoned control (0.75% ethylene glycol), and experimental (0.75% ethylene glycol and concentrations of 150, 300, and 500 mg/kg body weight of the extract) and were treated for 38 days. Then, the liver tissue of the rats was removed and transcriptional levels of NF-κB and MPO genes were evaluated by real-time PCR method.
The transcriptional level of the NF-κB and MPO in the liver of the poisoned control group rats has a significant increase compared to the healthy group. Treatment with hydroethanolic extract of C. melo seeds in concentrations of 300 and 600 mg/kg of body weight causes a significant decrease in the transcriptional level of the NF-κB gene in the experimental groups. The treatment of hydroethanolic extract of C. melo seeds in concentrations of 150, 300, and 600 mg/kg of body weight causes a significant decrease in the transcription of the MPO gene in the experimental groups compared to the poisoned control group.
The decrease in MPO and NF-κB indicates the therapeutic effect of melon seed extract on liver damage.
C. melo seed , liver toxicity , MPO , NF-κB
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