Effect of Lawsonia inermis extract on Chlorella vulgaris microalgae

The biofouling phenomenon causes great economic damage to artificial structures, so that by reducing the maneuverability of vessels and thus reducing their speed, it increases fuel consumption as well as increases maintenance costs. Today, many studies have been done to solve the problems of biofouling, the most important of which is to identify natural antifouling compounds. In this study, the anti-folling properties of henna (Lawsonia inermis) were investigated using n-hexane, ethyl acetate, methanol and aqueous. Treatments were performed in 3 replications. After 24 hours, under a light microscope, the number of algae was counted by a neobar slide. Data were analyzed using SAS software and data were compared by Duncan's multiple range test. Statistical analysis of henna extracts on Chlorella vulgaris showed that there was a statistically significant difference between the treatments at the probability level of 0.001. Examination of the results related to growth changes showed that with the application of treatments, the growth of this algae was significantly inhibited. Ethyl acetate extract with a concentration of 5 mg/ml, deoxygenate extract with a concentration of 20 mg/ml and methanolic extract with a concentration of 80 mg/ml and aqueous extract with a concentration of 160 mg/ml were the most inhibitory effect on the survival of Chlorella vulgaris.