For the purpose of this study, the antimicrobial effects of Zosima absinthifolia extract were tested on Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli). Dried seeds of Zosima absinthifolia plant (100 g) were powdered and soaked in a sufficient amount of water and the extraction process was done using a soxhlet apparatus. The obtained extract was then condensed in a rotary evaporator, transesterification process was applied and the resultant product was dehydrated completely.Using GC-GC/MS instruments, it was then analyzed. The extract contains essential ingredients including n-octanol, germacrene D, β-caryophyllene, α-pinene, limonene, octyl acetate, caryophyllene oxide and n-octanal. The extract was used to determine its antimicrobial effect. Culture medium was prepared and the test was performed by disk diffusion method. The preliminary results showed that the extract has antimicrobial effects on both bacteria. This suggests that the plant can be used in future as an effective substance in the treatment of infections caused by these bacteria.