Antimicrobial and Healing Effect of Nettle, Purslane and Hedge Nettle Extracts on Burn Infections of Staphylococcus aureus in Mice

Staphylococcus aureus is cause of hospital infections and infectious illnesses. Urtica dioica, Portulaca oleracea and Stachys schtschegleevii have more antimicrobial and healing effects. In this project, the antimicrobial and healing effects of plants extracts with silver sulfadiazine were studied on burn infections of Staphylococcus aureus in rats.

 Firstly, ethanolic and acetonic extracts of plants were prepared in the laboratory. Then, the MIC and MBC of the extracts were determined by the dilution method in the Muller Hinton broth. In study of animal model, firstly the bacteria were inoculated with a concentration of (5×105 CFU/ml) to the wound site on rats. After 24 hours, ointments were prepared based on MBC concentration from extracts of mentioned plants for 1g of silver sulfadiazine and was used to treatment.

 In studies conducted on rats, it was found that ethanolic and acetonic extracts of Urtica dioica, as well as the acetonic extract of Portulaca oleracea had more antimicrobial and healing effect on Staphylococcus aureus. But in the wound treated with ethanolic and acetonic extract of Stachys schtschegleevii, bleeding was seen. 

 Ethanolic and acetonic extracts of Urtica dioica and the acetonic extract of Portulaca oleracea had more antimicrobial and restoration effects on burn wound infection. Ethanolic and acetonic extract of Urtica dioica had better healing effects than acetonic extract of Portulaca oleracea. As a result, extract of Urtica dioica could be used in preparation of burn ointments.