Identification of Chemical Compounds in the Essential Oil of Salvia reuterana Bioss. and Evaluation of Antioxidant Properties and the Aamount of Phenols and Flavonoids in the Whole Plant Extract

Isfahan sage with the scientific name of Salvia reuterana belongs to the genus Labiatae and has a wide distribution in Iran. The genus Salvia is one of the important genera of the genus Labiatae. Because of the carcinogenic effects of synthetic antioxidants on the market, researchers are keen to replace them with natural compounds that have similar properties. Antioxidant compounds such as phenolic acids, polyphenols and flavonoids inhibit free radicals such as hydroperoxides and thus prevent the oxidative mechanism that leads to erosive diseases. In this study, antioxidant and antimicrobial activity of aerial parts of Isfahan sage by five tests including free radical scavenging activity (DPPH), iron ion chelating (FIC), determination of total phenolic content (TPC), total flavonoid content ( TFC) and antimicrobial test (DD assay) were evaluated. For this purpose, different parts of Isfahan sage plant including flowers and leaves were collected from Sabzevar in May 2014. The samples were dried, powdered and soaked in methanol solvent, and the crude extract was fractionated with n-hexane, water and ethyl acetate solvents, respectively. The water fraction of the flower in RSA (DPPH) test showed the highest activity with values of 6.35 ± 0.29 μ g / mL.The ethyl acetate part of the leaf had the highest TFC with 176.76 ± 1.51 mg RU / g and the aqueous ethyl acetate part of the flower had the highest TPC with 76.5 2 ± 2.07 and 68.95 ± 2 2.07 mgGEA / g. Antimicrobial test of ethyl acetate extract of leaves and flowers showed significant antimicrobial activity against Citrobacter frurdii and Pseudomonas aeruginosa, while little activity was observed against Serratio (-).