Identification of Baetis (Rhodobaetis) braaschi (Zimmermann, 1980) from Hablehrood River (Iran) using COI barcoding

Baetis (Rhodobaetis) braaschi is an Ephemeropteran species with a vast territory in the world with its eastern limit ends in Iran. This study was conducted to investigate the COI gene sequences of B. braaschi from Hablehrood River, Tehran, Iran. In this study, a molecular database of 780 bp was analyzed. The intraspecific genetic difference was found to be insignificant. The phylogenetic analysis identified that four haplotypes (58227, 23419, 28407, and 34719) from Hablehrood River highly supported clusters belonging to B. braaschi. Topology of the evolution tree on the basis of maximum likelihood method showed that the samples collected in this study were monophyletic with the samples recorded for the Czech Republic.