The Study of Genetic Diversity of Camels in North of Kerman Province Using F Statistics

Genetic variation among the individuals is considered as an important tool for conservation of livestock animals. Application of molecular markers to determine genetic variation between populations has been widely used in recent years. This study conducted to analyze genetic diversity using F statistics in populations of  Camelus dromedaries in north of Kerman using 8 autosomal microsatellite markers (YWLL08, VOLP03, VOLP08, YWLL38, CVR01, YWLL44, VOLP32 and VOLP67). Eighty-one blood samples were collected from Shahr-e Babak, Rafsanjan and Ravar. Total DNAs of the samples using salting out were extracted and applied for genotyping analysis. The result showed that the highest and the lowest allele number and effective alleles are shown in YWLL08 (21 and 14.9) and VOLP32 (4 and 3.11), respectively. Fixation index (FST) values for markers YWLL08, VOLP03, VOLP08, YWLL38, CVR01, YWLL44, VOLP32 and VOLP67 was obtained 0.036, 0.088, 0.080, 0.045, 0.054, 0.0698, 0.014 and 0.060, respectively. This result showed that differentiation is low between populations. The highest gene flow obtained between Shahr-e Babak population and Rafsanjan2 samples (15.83) and the lowest gene flow was observed between the two populations of Ravar (6.49). In general, it can be concluded that Camelus dromedarius in north of Kerman has approximately high genetic diversity and microsatellite markers have approximately high polymorphism and therefore can be used for genetic studies.