Association of rs2787094 in ADAM33 and rs11650354 in TBX21 Gene Polymorphisms with Asthma in Iranian Patients

Asthma is a common inflammatory disease of the airways which is characterized by variable and recurrent symptomes. The disease causes airways inflamtion and hyperresponsiveness, reversible airways obstruction and airway deformity and  accomponied with symptoms including shortness of breath, cough and wheezing in patients. Environmental and genetic factor  such as gene polymorphisms are involved in the development of asthma. Gene polymorphisms alter the function of genes, hence they involve in disease progression. Studies have shown genetic polymorphism in ADAM33 and TBX21 genes are associated with the risk of asthma.

In present study the polymorphism rs2787094 of ADAM33 and rs11650354 of TBX21 genes was assessed for the first time in Iranian asthma patients and healthy individuals. For this purpose, 30 blood samples from asthma patients and 30 samples from healthy donors was collected. Then DNA was extracted, and subsequently ARMS-PCR technique was used, applying specific designed primers for normal and mutant allels for each polymorphism of candidate genes and then the results were analysed.

The results indicated rs2787094 polymorphism in ADAM33 was significantly associated with the risk of asthma, while there was no significant relation between rs11650354 in TBX21 with asthma risk.

It seems that rs2787094 polymorphism in ADAM33 and different genotype in this locus play siginificant role in asthma risk in Iranian patients.