The Effect of Aerobic Exercise Training and Consumption of Curcumin Nano Micelles on the Expression Level of CASP3, CASP9, Bax and BCL2 Genes on Cardiac Tissues of Balb/C Mice with Induced Breast Cancer Treated with Doxorubicin

Doxorubicin is a pervasive drug in cancer treatment, but its consumption is restricted due to various side effects in the body tissues. Therefore, this research aimed to reduce the side effects of this drug through the impact of a period of aerobic training along with the consumption of curcumin nano micelles on the expression level of CAS3, CAS9, BCL2, and BAX genes in cardiac tissues of Balb C mice with breast cancer induced by 4T1 cell line.

24 female Balb/C mice were randomly assigned to four control groups:  treated with doxorubicin, treated with doxorubicin and aerobic exercise training, treated with doxorubicin and nanocurcumin and bimardoxorubicin supplement, aerobic exercise training, and supplement. The 4T1 cell line, arising mouse mammary tumor, was used in this study. Doxorubicin (2mg/kg) was injected subcutaneously six times on days 28, 21, 14, 7, 1, and 35.

Aerobic exercise training significantly decreased the expression level of CAS3, CAS9 and BAX genes, while it had no significant effect on the level of BCL2 (P≤ 0/05). Nanomicelle curcumin supplement caused a significant decrease in the expression of CAS3 and CAS9 genes, but it had no significant effect on the BAX gene expression. However, it caused a significant increase in BCL2 gene expression (P≤ 0/05).

It can be concluded that aerobic exercise training and consumption of Nano micelles containing curcumin as a therapeutic supplement alone or simultaneously reduced sensitivity to cardiac apoptosis and protected non-target tissues in the heart during doxorubicin therapy. Doxorubicin (DOX) inhibits the growth of tumor cells and improves the effectiveness of cancer treatment.