Optimizing the green synthesis formulation of silver nanoparticles using Cherry tree leaf extract and evaluation its stability

Many researchers have reported the synthesis of metal nanoparticles by plant extracts and their potential applications. Cherry tree leaf extract is one of the effective materials in the synthesis of AgNPs, which is confirmed by the results of FTIR analysis. Did AgNPs according to the design of the experiment carried out by the Mixture Design method by mixing three regenerating substances (cherry tree leaves 0-3 ml), basic salt substance (1 mM solution of AgNO3 5-9 ml) and stabilizing agent (AloeVera gel) (0-1 ml) was synthesized by green method. After the optimization, the results showed that the amounts of 2.51, 5.79 and 0.69 mL respectively for reducing agent, basic solution and stabilizing agent were the most suitable mode of synthesis of nanoparticles, and in this mode, the highest concentration of 16.88 ppm, the lowest average particle size of 36 nm, the most appropriate PDI of 0.43 and the highest zeta potential of 25 mV will be obtained. The properties of synthesized AgNPs were evaluated in optimal conditions and the results of UV-Vis and DLS analyzes confirmed the obtained results with slight differences. AgNPs showed good antioxidant properties of 28.45% and antibacterial properties against Gram-positive (Staphylococcus aureus) and Gram-negative (Escherichia coli) bacteria. Antifungal properties were also found to be 65.55%.