Detection of White Spot Virus in Wild Sources of Boyrat Shrimp Breeding Site in Bushehr Province

Shrimp diseases are one of the major limitations for increasing shrimp production, and the cause of white spot disease is the most important pathogenic virus in shrimps, which causes irreparable damage to the shrimp farming industry. Therefore, the purpose of the present study was to trace the white spot virus in wild sources at the Boyrat shrimp breeding site in Bushehr province. The present study was performed in Boyrat shrimp breeding complex of Bushehr province by sampling of wild resources. DNA was extracted from 20 samples of wild resources collected included: three samples of Metapenaeus ensis, one sample of Saurida tumbil, one sample of Terapon puta, ten samples of mud crab, two samples of blue crab and two samples of Litopenaus vannamei post larvae during preparation, grow out and harvesting from ponds and white spot virus was detected with PCR test. The results obtained in the present study indicated that the most positive cases are related to grow out stage (83.3 %) and harvesting stage (28.6 %) and in the preparation phase, the frequency of the disease was 0%. In total, 35% of cases (Metapenaeus ensis, small blue crab and small mud crab) were infected with white spot disease in all cases. The highest frequency of infection with the mentioned virus was in Metapenaeus ensis (100 %), small blue crab (100 %), and small mud crab (33.3 %), respectively. In total, among all studied species, 25% infection with white spot disease was observed. According to the results obtained in the present study, in order to prevent pollution in shrimp ponds, it is recommended to use different management methods to prevent wild sources from entering the ponds.