Antibacterial and Antibiofilm activity of P. Pentosaceus Bacteriocin (Pediocin) Isolated from Cheese and its Optimization for the Bacteriocin Production
Article Type:
Research/Original Article (دارای رتبه معتبر)
The objective of the present study was to assess the antibacterial and antibiofilm activities of Pediocin, a bacteriocin extracted from cheese, versus a subset of bacteria linked to foodborne diseases. The agar well diffusion method was used to evaluate the antibacterial activity of Pediocin. The results showed that Pediocin had a potent bactericidal effect against all the tested bacterial strains. The crystal violet staining method was used to assess Pediocin's antibiofilm efficacy, and the results showed that Pediocin significantly inhibited the development of biofilms by the tested bacterial strains. Investigating the effects of temperature, pH, and medium make-up improved Pediocin output. It was discovered that a temperature of 30 °C, a pH of 6.5, and a medium made up of tryptone, yeast extract, and glucose were the ideal conditions for the creation of pediocin. Pediocin demonstrated significant antibacterial activity against all the tested bacterial strains, with the highest activity against Staphylococcus aureus, Streptococcus pyogenes and Helicobacter pylori. The minimum inhibitory concentration (MIC) values of Pediocin against the tested bacterial strains varied from 0.5 to 32 µg/mL. Among them, the lowest MIC was observed against S. aureus, P. aeruginosa, and K. pneumoniae. Similarly, the minimum bactericidal concentration (MBC) values ranged from 2 to 64 µg/mL, with the lowest MBC observed against S. aureus, P. aeruginosa, and K. pneumoniae. The large-scale manufacture of Pediocin, which may be utilized as a natural food preservative to stop the growth of pathogenic bacteria and lower the frequency of foodborne diseases will be made easier by the optimized production circumstances. The potential of Pediocin for food preservation and the security of its use require more research.
International Journal of Advanced Biological and Biomedical Research, Volume:11 Issue: 3, Summer 2023
134 to 150  
دانلود و مطالعه متن این مقاله با یکی از روشهای زیر امکان پذیر است:
اشتراک شخصی
با عضویت و پرداخت آنلاین حق اشتراک یک‌ساله به مبلغ 990,000ريال می‌توانید 70 عنوان مطلب دانلود کنید!
اشتراک سازمانی
به کتابخانه دانشگاه یا محل کار خود پیشنهاد کنید تا اشتراک سازمانی این پایگاه را برای دسترسی نامحدود همه کاربران به متن مطالب تهیه نمایند!
  • حق عضویت دریافتی صرف حمایت از نشریات عضو و نگهداری، تکمیل و توسعه مگیران می‌شود.
  • پرداخت حق اشتراک و دانلود مقالات اجازه بازنشر آن در سایر رسانه‌های چاپی و دیجیتال را به کاربر نمی‌دهد.
In order to view content subscription is required

Personal subscription
Subscribe for 50 € euros via PayPal and download 70 articles during a year.
Organization subscription
Please contact us to subscribe your university or library for unlimited access!