Alteration of Neurodevelopmental Gene Expression Following Prenatal Exposure to Aquatic Crocus Sativus L. Extract in Mice
Message:
Article Type:
Research/Original Article (دارای رتبه معتبر)
Abstract:
Background
Crocus sativus (saffron) is used since ancient times as a medicine and spice. Various studies have demonstrated its antioxidant, anticancer, and anti-inflammation properties. In folkloric Iranian medicine, saffron is known as an abortifacient agent and some investigations announced it as a teratogen. The current study aimed to investigate the C. sativus extract effect on the fetal brain.
Methods
The NMRI female mice were randomly divided into the control group and three saffron aquatic extract treated groups (25, 50, and 100 mg/kg of saffron aquatic extract). The fetuses were treated during 7-12 days post coitum (dpc). The fetuses were morphologically evaluated. Fetal brain tissues were investigated by histology and real-time PCR for Foxg1, Foxa2, Wif1, and Fgf8 expression.
Results
We found that three treatments reduced the number of fetuses. Fetuses of 25 mg/kg treated were significantly heavier (P<0.001) and had shorter tails (P<0.001) than controls. No difference was observed among treated and control groups in histological prospect. Foxa2 and Wif1 expressions dose-dependently increased (P<0.0001), while Foxg1 mRNA level increased in 25 mg/kg treatment (P<0.0001). Fgf8 expression decreased significantly in 25 mg/kg and 50 mg/kg treatments (P<0.0001 and P<0.001, respectively).
Conclusion
These findings suggested that although no difference was observed in the histology of the fetal brain, the alteration of mentioned genes could affect the cellular biochemistry, molecular structures, and cell types in the developing brain.
Language:
English
Published:
International Journal of Advanced Biological and Biomedical Research, Volume:11 Issue: 3, Summer 2023
Pages:
172 to 184
magiran.com/p2623572  
دانلود و مطالعه متن این مقاله با یکی از روشهای زیر امکان پذیر است:
اشتراک شخصی
با عضویت و پرداخت آنلاین حق اشتراک یک‌ساله به مبلغ 990,000ريال می‌توانید 70 عنوان مطلب دانلود کنید!
اشتراک سازمانی
به کتابخانه دانشگاه یا محل کار خود پیشنهاد کنید تا اشتراک سازمانی این پایگاه را برای دسترسی نامحدود همه کاربران به متن مطالب تهیه نمایند!
توجه!
  • حق عضویت دریافتی صرف حمایت از نشریات عضو و نگهداری، تکمیل و توسعه مگیران می‌شود.
  • پرداخت حق اشتراک و دانلود مقالات اجازه بازنشر آن در سایر رسانه‌های چاپی و دیجیتال را به کاربر نمی‌دهد.
In order to view content subscription is required

Personal subscription
Subscribe magiran.com for 50 € euros via PayPal and download 70 articles during a year.
Organization subscription
Please contact us to subscribe your university or library for unlimited access!