Antibacterial effect of silver nanoparticles synthesized by green method using diospyros extract against the orodental bacteria
Dental caries is the most widespread chronic disease.There are several medical procedures to prevent and control its extension.Based on side effects of chemical products and effectiveness of nanoparticles, this study done inorder to evaluate the antimicrobial effect of silver nanoparticles produced by green synthesis using diospyros extract on oral bacteria.
In this experimental study the diospyros extract was produced by Maceration procedures at Zanjan university of medical sciences in 2019 .The effect of silver nanoparticles produced by green synthesis was determined on various concentrations of Enterococcus, Streptococcus Mutans, Streptococcus Subrinus, Streptococcus Salivarius, Lactobacillus micro organisms by cap plate method. Minimum Inhibitory Concentration (MIC) and Minimum Bacterial Concentration (MBC) factors was determined. The datas was imported to the SPSS 24 program and analysed by Wilcoxan , Mann-Withny, Kruskal-wallis tests.
.the MIC factor for streptococcus mutans, enterococcus, streptococcus salivarius was determined 1024 μgr⁄mL and for streptococcus subrinus and lactobacillus 512 μgr⁄Ml. Although the antimicrobial resistance due to MBC factor is decreased from Enterococcus to, streptococcus mutans, lactobacillus, streptococcus salivarius, streptococcus subrinus sequentially (P=0.014). The finding showed that the antimicrobial resistance of streptococcus mutans, enterococcus, streptococcus salivarius due to MIC factor is more than streptococcus subrinus and lactobacillus.
The results showed the bacteriostatic effect of silver nanoparticles produced by green synthesis using Diospyros extract on oral pathogens.due to herbal source an being native, maybe this plant can be useful to produce mouthwashes and toothpastes.
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