Ajowan, Biological Elicitor, Non-Biological Elicitor, Thymol

Carum copticum is as an herbal medicine from the family Apiaceae with compounds such as thymol, cement, alpha-pinene, gametropin, betapinene, micron, and carvacrol in the treatment of angina, kidney stones, asthma, rheumatism, influenza that used as a strong disinfectant and boost digestion. In this study, expression of beta-amyrin acid synthase (beta-Amirinsanthase) under different treatments of silver nanoparticles (0, 30, 60, 90 ppm), chitosan (0, 100, 150, 200 ppm) and populations (Sistan and Pakistan) was investigated. Solubility was carried out in the quaternary stage at 48 hours and 72 hours. The results of analysis of variance showed that the application of chitosan eclipses and silver nanoparticles caused a change in the beta-amyrein gene expression pattern. The highest expression, 48 hours after treatment, was observed with just about 150 ppm of chitosan. By increasing chitosan concentration, a significant decrease occurred in the expression of the gene for 72 hours while treatment with 90 ppm of silver nanoparticles in 48 hour after treatment has the most gene expression. In the effects of chitosan (150ppm) and silver nanoparticles (90ppm) based on 48 hours after treatment was observed the most gene expression for both populations. Overall, the results of this study showed that chitosan and silver nanoparticle elicitors had a positive effect on beta-amyrein gene expression, but the highest expression was obtained in Pakistan population at 48 hours intervals.