Major flavonoids identification by HPLC-MS/MS and evaluation of antiradical potential of mung bean (Vigna radiata) seed extracts in Burkina Faso

Article Type:
Research/Original Article (دارای رتبه معتبر)

Mung bean is one of the most essential legumes in Burkina Faso due to its short maturation cycle (45 to 55 days) and its nutritional content. This work aims to contribute to a better knowledge of the bioactive compounds of the seeds of Beng Tigré, a mung bean cultivar originating from India and acclimatized in Burkina Faso. To do this, the contents of total phenolic compounds, flavonoids, and carotenoids were determined by colorimetric methods. The mineral elements were assayed by atomic absorption spectrometry after the seeds mineralization. The antiradical potential was evaluated by monitoring the disappearance of the stable radical DPPH• by UV-Visible absorption spectrophotometry at 517 nm and compared to standards. Major flavonoids were identified by high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). Total phenolic compounds, flavonoids, and carotenoids were 4. 356±0.162 mg GAE/g, 3. 151±0.262 mg QE/g, and 0.302±0.012 mg BCE/g, respectively. Potassium, magnesium, and calcium were, respectively, the most abundant mineral elements in the seeds: K > Mg > Ca > Fe > Zn > Na > Mn > Cu. An antiradical activity of the Beng Tigré extract was observed with an IC50 of 209. 97±13. 55 µg of AO/g DPPH•. This activity is less effective than Gallic acid, ascorbic acid, and trolox. Finally, the HPLC-MS/MS analysis identified four compounds: naringenin 8-C-glucoside, luteolin 8-C-glucoside, apigenin 6-C-glucoside, and luteolin 7-O -rutinoside. Due to their high biological potential, these so-called bioactive compounds are the subject of growing research interest.

Eurasian Chemical Communications, Volume:5 Issue: 10, Oct 2023
935 to 952  
دانلود و مطالعه متن این مقاله با یکی از روشهای زیر امکان پذیر است:
اشتراک شخصی
با عضویت و پرداخت آنلاین حق اشتراک یک‌ساله به مبلغ 1,390,000ريال می‌توانید 70 عنوان مطلب دانلود کنید!
اشتراک سازمانی
به کتابخانه دانشگاه یا محل کار خود پیشنهاد کنید تا اشتراک سازمانی این پایگاه را برای دسترسی نامحدود همه کاربران به متن مطالب تهیه نمایند!
  • حق عضویت دریافتی صرف حمایت از نشریات عضو و نگهداری، تکمیل و توسعه مگیران می‌شود.
  • پرداخت حق اشتراک و دانلود مقالات اجازه بازنشر آن در سایر رسانه‌های چاپی و دیجیتال را به کاربر نمی‌دهد.
In order to view content subscription is required

Personal subscription
Subscribe for 70 € euros via PayPal and download 70 articles during a year.
Organization subscription
Please contact us to subscribe your university or library for unlimited access!