Investigating Common Mutations in Exon 20 and 21 of EGFR Gene in Lung Cancer Tumor Tissue of NSCLC Type

Article Type:
Research/Original Article (دارای رتبه معتبر)

 A mutation in the EGFR gene can be a marker for non-small cell lung cancer (NSCLC). The frequency of this mutation is very different in various countries and ethnic groups.


 This study investigated the frequency of mutations in exons 20 and 21 of the EGFR gene in patients with NSCLC in Iran.


 Twenty paraffin blocks with an average age of 3 years were prepared from the Tehran Cancer Institute. The samples belonged to 5 women and 15 men aged 61 - 74 years and were confirmed by a pathologist in terms of positivity and type of tumor. After deparaffinization, DNA extraction was performed using a QIAGEN kit. The appropriate probes and primers were designed, and finally, the samples were examined by the real-time TaqMan method, and the data were analyzed.


 The findings showed that 15% and 10% of patients had mutations in exon 20 and 21 of the EGFR gene, respectively. The T790M mutation of exon 20 was observed in three patients (tumors of adenocarcinoma type and large cell cancer), and L858R and L861Q mutations of exon 21 of the EGFR gene were observed.


 The frequency of mutation in exon 20 and 21 of the EGFR gene in NSCLC patients among Iranians is close to Middle Eastern and European patients, but it is very different from the frequency of this mutation among East Asian and North Asian patients.

Gene, Cell and Tissue, Volume:11 Issue: 1, Jan 2024
دانلود و مطالعه متن این مقاله با یکی از روشهای زیر امکان پذیر است:
اشتراک شخصی
با عضویت و پرداخت آنلاین حق اشتراک یک‌ساله به مبلغ 1,390,000ريال می‌توانید 70 عنوان مطلب دانلود کنید!
اشتراک سازمانی
به کتابخانه دانشگاه یا محل کار خود پیشنهاد کنید تا اشتراک سازمانی این پایگاه را برای دسترسی نامحدود همه کاربران به متن مطالب تهیه نمایند!
  • حق عضویت دریافتی صرف حمایت از نشریات عضو و نگهداری، تکمیل و توسعه مگیران می‌شود.
  • پرداخت حق اشتراک و دانلود مقالات اجازه بازنشر آن در سایر رسانه‌های چاپی و دیجیتال را به کاربر نمی‌دهد.
In order to view content subscription is required

Personal subscription
Subscribe for 70 € euros via PayPal and download 70 articles during a year.
Organization subscription
Please contact us to subscribe your university or library for unlimited access!