Inhibitory Effect of Sea Cucamber, Holothuria parva Extract on Activity of Alpha -Amylase

Alpha-amylase enzyme inhibitors prevent the conversion of polysaccharides into monosaccharides. Inhibiting this enzyme from the absorption of simple sugars in the digestive system and thus preventing the increase in blood glucose levels has been effective. The purpose of this study is to investigate the effect of ethyl acetate, methanol and water-methanol extracts of Holothuria parva on alpha-amylase enzyme inhibition. In this research, first extraction was done from the body wall, intestines and viscera of Holothuria parva based on increasing polarity, and in the next step, alpha-amylase enzyme activity was determined in the presence of different extracts under enzyme activity measurement conditions by DNSA colorimetric method, and IC50 were obtained and compared with the amount of acarbose as a positive control. In this study, the results showed that the percentage of inhibition increased with the increase in the concentration of the extracts and among all the extracts, the highest inhibitory percentage related to acarbose at a concentration of 0.8 mg/ml equals 96.92% and the lowest amount related to the water-methanol extract of the body wall at a concentration of 0.2 mg/ml equals 61.79% and the inhibition percentage of acarbose in both body parts is higher than other extracts (IC50=88.18-91.74 mg/ml). Considering the inhibitory effect of extracts extracted from Holothuria parva species on alpha-amylase enzyme, in the future, it can be used in the field of producing antidiabetic drugs of marine origin with minimal adverse side effects.