Evaluation of Antioxidant Effect of Cerium Oxide Nanoparticles on Adipose Tissue Derived Mesenchymal Stem Cell Line

Today, the use of nanoparticles in various fields is increasing. Cerium oxide nanoparticles are one of the types of nanoparticles that are widely used in the nanomedicine as an antioxidant. The specific antioxidant property of cerium oxide nanoparticles made us investigate its antioxidant effect on mesenchymal stem cell line derived from adipose tissue. After preparation and passage of mesenchymal stem cells derived from adipose tissue, these cells were tested for oxidative stress with different concentrations of hydrogen peroxide, which was checked by MTT test, treated and the amount of free radical ROS was measured. Then, the cells were treated with different concentrations of cerium oxide which checked by MTT test and the cell survival investigated by Annexin 5-propidium iodide test by flow cytometry to check apoptosis and finally the results were statistically analyzed. became. The greatest effect of hydrogen peroxide oxidative stress was calculated in 24 h at a concentration of 200 μM and the results of MTT were calculated to determine the IC50 of cerium oxide nanoparticles at a concentration of 100 μM/ml in 24 h. The results showed that this nanoparticle is not only non-toxic for these cells, but also increases their proliferation in certain concentrations. The results of the flow cytometry tests and the evaluation of the effects of cerium oxide nanoparticles also showed that these nanoparticles have a good antioxidant capacity and the survival of cells treated with cerium oxide was similar to the group treated with vitamin C. This shows the effective and beneficial effects of cerium oxide nanoparticles and the potential of this compound in cell regeneration and survival. Based on these results, cerium oxide can be an improving factor in cell proliferation and cell therapy.