Investigating the effect of intramyocardial injection of DNAzyme-containing nisosome of NF-κB gene on the level of cardiac tissue NF-κB protein expression in cardiac ischemia-reperfusion of male rats

NF-κB factor is activated by ischemic damage and reperfusion and has a crucial role in transcription of several genes. Applying DNAzyme is one of the novel therapeutic means. Niosome is a new drug delivery system that is controllable ae used targeted. Applying DNAzyme-containing nanoniosome to diminish the expression of NF-κB protein after cardiac ischemia.

In the current study, twenty-five male rats were assigned into 5 groups: 1- Control group, 2- Ischemic induced group, 3- Post ischemic DNAzyme receiving group, 4- Post ischemic DNAzyme-bearing nanoniosome receiving group, and 5- Post ischemic nanoniosome receiving group. After 72 hours, western blotting was performed on rats’ hearts to assess expression of NF-κB protein in treated groups.

post ischemic treatment with DNAzyme-bearing nanoniosome led to considerably decrease of NF-κB expression compared to ischemic rats (P<0.05).

Results demonstrated the efficiency of DNAzyme-containing nanoniosome on NF-κB protein after cardiac ischemia. Therefore it is propounded as a curative method for cardiac ischemia