Investigation the effect of putrescine, IBA and wounding on rooting of cutting of Rosa hybrida cv. Sanaz-e-Zard under greenhouse and in vitro conditions

Miniature roses are hybrid roses that are widely used in landscape or as pot plants and cut flowers. Traditional methods of propagation for this species is associated with time limitation and rootstock propagule, and micropropagation techniques are increasing. Root induction of roses cuttings through different plant growth regulators is important. In this study, the effects of different concentrations of Indole-3-Butyric Acid (IBA), putrescine and wounding the end of cuttings on rooting of Rosa hybrida cv. SanaZard under in vitro and greenhouse conditions was investigated. Micro-cuttings were planted in MS basal culture medium containing five concentrations (0, 0.25, 0.5, 1 and 2 mg L-1) of IBA and putrescine. In the greenhouse, the cuttings were treated with five levels of IBA, putrescine (0, 0.25, 0.5, 1 and 2 g L-1), and two levels of wounding (with and without wounding) and planted in pots after application of treatments. This study was conducted as a factorial experiment in a completely randomized design with three replicates. The results of greenhouse experiments showed that the maximum length and number of roots were obtained under the treatment of 1 g L-1of IBA (31.5 cm and 6.50, respectively). Wounding the end of the cutting increased the number of roots by 33.33%. In in vitro condition, the highest number of roots was observed in 1 mg L-1 IBA + 2 mg L-1putrescine (6) treatment and the highest number of shoots were obtained with the simultaneous application of 0.25, 0.5 and 2 g L-1 of IBA + all levels of putrescine. In general, it seems that the combined use of IBA and putrescine could increase the rooting of miniature rose cuttings in in vitro and in situ conditions. Also, wounding the ends of cuttings plays an important role on inducing the roots under greenhouse environment.