The investigation of death-inducing potency of a recombinant Adenovector expressing Mda-7-tlyp-1 on different cancer cell lines

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Article Type:
Research/Original Article (دارای رتبه معتبر)
Abstract:
Introduction

Mda-7 could complement cancer gene therapy and tethering tumor-homing peptides (THPs) to the structure might improve the therapeutic efficacy. Herein, an Adenovector expressing Mda7-tLyp1 (Ad-Mda7-tLyp1) was prepared and evaluated on breast, liver and gastric cancer cell lines.

Materials and Methods

 After preparation of recombinant Ad-Mda7-tLyp1 and Ad-Mda7, the expression of recombinant proteins was analyzed by ELISA. Adenovectors were transduced (MOI=2-5) into Hep-G2, MCF7, MKN-45, and normal fibroblast, then tumor killing effect was measured by cytopathic effect monitoring, MTT viability test, BAX gene expression analysis, and Caspase3/7 colorimetric assay.

Results

 The ELSA revealed a sustained level of recombinant proteins secretion following vector transduction. The CPE and viability test showed that Ad-Mda7 and Ad-Mda7-tLyp1 have a significant killing effect on MCF-7, Hep-G2 and MKN-45 compared to fibroblast cell. The BAX expression analysis and caspase 3/7 activity was also showed a significant increase(P<0.05) in apoptosis level following Adenovector transduction into cancer but not fibroblast cells.

Conclusion

 The newly constructed Ad-Mda-tlyp1 showed a suitable tumor cell killing activity and either enough specificity.

Language:
English
Published:
Gastroenterology and Hepatology From Bed to Bench Journal, Volume:17 Issue: 1, Winter 2024
Pages:
45 to 56
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