Preparation and evaluation of sodium alginate nanoparticles containing CRM197 protein by ionic gelation method

Article Type:
Research/Original Article (بدون رتبه معتبر)

Development of the recombinant vaccines against infectious diseases is dependent on the identification of immunogenic antigens and vaccine delivery systems such as polymeric nanoparticles that are able to stimulate immune responses similar to or better than conventional vaccines and reduce complications associated with traditional vaccines. At the present study, synthesis and properties of the sodium alginate nanoparticles carrying CRM197 protein as an antigen delivery system were evaluated. Synthesis of the blank optimized without protein loading and protein-containing nanoparticles was performed by ionic gelation method. After designing of the experiment (DoE) and determining the influential physicochemical factors in ideal nanoparticles synthesis; size, zeta potential, morphology, encapsulation efficiency, release pattern and FT-IR spectroscopy were investigated. The optimized nanoparticles were prepared at a concentration of 0.2% w/v sodium alginate, 0.1% w/v calcium chloride, 0.04% w/v poly-L-lysine during 45 minutes of stirring at 2000 rpm and in pH 6.5. The average nanoparticle size for blank and CRM197 loaded nanoparticles were 88 and 245 nm also zeta potential -21 and -24.2 mV, respectively. LE and LC were >80% and >20%, respectively, associated with a stable and long-term encapsulated protein release pattern from nanoparticles. Absence of local and systemic signs, as well as weight gain in the mice group studied, indicated the safety of the nanoparticles and CRM197 protein combination. Based upon the above achievements, alginate nanoparticles can be used as an antigen delivery system for targeted delivery with controlled, slow release and improved stability of recombinant diphtheria antigen (CRM197) for immunization against diphtheria disease.

Journal of Chemical Research and Nanomaterials, Volume:2 Issue: 3, 2024
55 to 70  
دانلود و مطالعه متن این مقاله با یکی از روشهای زیر امکان پذیر است:
اشتراک شخصی
با عضویت و پرداخت آنلاین حق اشتراک یک‌ساله به مبلغ 1,390,000ريال می‌توانید 70 عنوان مطلب دانلود کنید!
اشتراک سازمانی
به کتابخانه دانشگاه یا محل کار خود پیشنهاد کنید تا اشتراک سازمانی این پایگاه را برای دسترسی نامحدود همه کاربران به متن مطالب تهیه نمایند!
  • حق عضویت دریافتی صرف حمایت از نشریات عضو و نگهداری، تکمیل و توسعه مگیران می‌شود.
  • پرداخت حق اشتراک و دانلود مقالات اجازه بازنشر آن در سایر رسانه‌های چاپی و دیجیتال را به کاربر نمی‌دهد.
In order to view content subscription is required

Personal subscription
Subscribe for 70 € euros via PayPal and download 70 articles during a year.
Organization subscription
Please contact us to subscribe your university or library for unlimited access!