Design and Developed Novel and Ultrasensitive Chemosensor for Sequential Determination of Arginine and Glutathione Amino Acids

In this study, a novel mixed ligand (Dithizone-Co(II)-Alizarin red S: DTZ-Co-ALR-) ensemble is designed and developed ultra-sensitive, and highly selective to UV-Vis absorption and for naked-eye detection of Arginine (Arg), and Glutathione (GSH). The out coming high sensitivity and selectivity for new receptor (DTZ-Co(II)-ALRs-Arg) was attained by adding Glutathione. The indicator is released due to its displacement from the mixed ligand (DTZ-Co-ALRs-Arg) by Glutathione and the change in absorbance may be due to the further complexation of GSH with the additional coordination sites present in the Arg bonded with the mixed ligand (DTZ-Co-ALRs). The label-free DTZ-Co-ALRs receptor provided sensitive and selective detection of L-Arginine, and Glutathione with detection limits of 0.03, and 0.009µmol L-1, respectively. The protocol especially offers high selectivity for the determination of Arg, and GSH among amino acids found in real samples. Moreover, the investigation of the logic behavior of the proposed DTZ-Co-ALRs receptor indicated its capability of functioning as an INHIBIT-type colorimetric chemosensor with the chemical inputs and UV-Vis absorbance signal as the output. This mixed ligand receptor could also behave as a molecular “keypad lock” with the correct sequential addition of Arg and GSH inputs.