Effect of Agar, Glucose concentration, Number of Bacteria and Atmospheric condition on growth of bacteria in the Gel Stabilized System

There are several methods for studying of microorganisms in the laboratory conditions that they mimic natural conditions. One of these methods is Gel Stabilized System. In this System microorganisms grow in the semisolid layer and form aria with very condensed bacterial population which appears like a Band and it is called Growth Band. Gel Stabilized System consists of two layers or phases that made of PYS Medium (Peptone Yeast extract Salt Solution Medium) with these differences: Glucose and 1.5% agar at the solid layer and 0.75% agar and certain number of bacteria in semisolid layer. H.pylori, L.plantarum and L.casei- casei grown in this system and produced Growth Band. The growth band formed in the condition of 2% Glucose to 10%, Different atmospheric conditions and height of semisolid phase, various agar concentrations and when the number of inoculated bacteria was over 10 × 105 /mL of medium culture. Ordinarily H.pylori formed 3 growth band and Lactobacilli 2.Three species are able to produce Growth Band in semisolid phase. The Growth Bands were formed depends on Glucose concentration, atmospheric conditions, height of semisolid phase and the number of inoculated bacteria but not to agar concentration in semisolid layers. We suggest for studying any substance or purpose is better the usage of a system with the following properties: 1) 2% glucose at solid phase 2) Aerobic atmospheric condition 3) 0.75% agar at semisolid phase 4) 50 mm semisolid phase height and 5) Number of bacteria inoculated over 3 × 105.