The investigation of phosphatidylinositol 3-kinase (PI3K) isoforms which express by human prostate cancer cell lines, PC3 and DU145

From Two highly metastatic prostate cancer cell lines, DU145 expresses PTEN gene while PC3 is null for it. PTEN is a tumor suppressor gene whose primary function is lipid phosphatase herewith PTEN antagonizes the PI3K activity. Phosphatidylinositol 3- kinase (PI3K) is involved in modulating basement membrane’s protein degradation, angiogenesis and neovascularization making possible cellular migration. There are several articles reporting up-regulation of PI3K gene in different kinds of metastatic cancers. Since despite of bearing functional PTEN, DU145 cell line manifest highly metastatic potential (as well as PC3 cells) it was interesting for us to know if there was any differences in PI3K isoforms expression patterns between these two cell lines. Total RNA was extracted from the cells and mRNA content was analyzed (through three different reverse transcriptase enzymes) using RT-PCR method. Unexpectedly data showed both of the cell lines express identical isoforms. Here, as the first report, we introduce P110α catalytic subunit and P85 adapter protein from class IA, PI3K-C2 from class II and Vps34p from class III of PI3K super family as PI3K isoforms which expressed by PC3 and DU145 cells. We propose to search for DU145 metastatic potential in inequality of PI3K isoforms supply. In this regard we are going to quantify each isoform mRNA individually for both PC3 and DU145 cells using Real-Time RT-PCR method.