Biocompatibility evaluation of Minalux and VeraBond2 in-vitro

Statement of Problem: One of newly presented base metal alloys (Minalux) is produced according to VeraBond2 alloy (Ni- Cr base) composition. Several studies showed that, cytotoxicity of base metal alloys can be occurred due to corrosion and element release. This study evaluated the biocompatibility of these two base metal alloys in three steps: as cast, after polishing and after porcelain firing cycles. Release of Ni and Cr ions were measured to determine if there is any difference between these two alloys. Samples of two base metal alloys were subjected to Neutral Red Assay, MTT Assay and Trypan Blue for biocompatibility tests. Fibroblast Balb/c 3T3 cells were used for cell culture. Samples were contacted directly with cells in 37ºc and 5% Co2 concentration for 72 hours. Teflon samples were used as negative control. ANOVA test was used to compare different groups of two alloys. In addition, the release of Ni and Cr ions in to saline solution was measured by means of atomic absorption spectrometry. MTT and Trypan Blue didn’t show any significant difference between Minalux, VeraBond2 and Teflon. Neutral Red Assay showed no significant difference between these two base metal alloys but as cast group was higher in cytotoxicity in comparisons with polished and firing groups in both two alloys. Release of Cr ion was non detectable (Cr < 1 PPB) but Ni ion was measured and Ni release was significantly different in as cast groups (P=0.007) of two alloys. There is no significant difference between cytotoxicity of two base metal alloys and polishing and firing can decrease cytotoxicity of both alloys.