Stereologic study on the acute effects of coffee extract on the kidney glomeruli in male rats

Background and Çoffes is a popular traditional drink in many countries, it has duretic and vasodilator properties and reduces free radicals of oygen. Ëvidences show that coffee reduces urea and serum keratinin, but over drinking leads to glomerulosch lerosis. Ïn this study the effects of high concentrations of coffee extract on kidney is studied by sterologic methods. Ïn this study 60 sprague-dawley rats weighing 230-250 grams were randomly selected and divided into 5 groups. To the control group water and to the experimental groups aqueous extract of coffee with concentrations of 0.025, 0.5, 1,1.5 gr/kg were administered oraly twice a day. Âfler 48 hours, the animals went under deep anaesthesia with ether, disected and after fiation with 10% formalin for solution by vascular perfured. Following routine tissue processing, 1 mm kidney tissue section was prepared and from each section, pairs of 5 μm diametor were prepared and stained with M H & Ë. prepared sections were studied by physical dissector method. There was no significant differece between the number of kidney glomeruli between the control and experimental groups. Çonclusion: Ït seems that, high concentrations of coffee has no effect on the number of glomeruli. Howere, further studies with unea and serum keratinin measurements are required to confirm the resuto reportes here.