Evaluation of adhesion and morphologic changes of human osteoblasts exposed to gray MTA, white MTA and Portland cement

Osteoblasts and periodontal ligament cells play a major role in wound healing after root end resection. The interaction of osteoblasts with filling materials is critical in healing of surgical lesions. The aim of this study was to evaluate the morphology and adhesion of human osteoblasts (MG-63 cell line) in contact with IRM, gray MTA, white MTA and Portland cement (PC) as root end filling materials. In this in vitro study, human osteoblasts of osteosarcoma were provided from the cell bank of Iran Pasteur Institute, and cultured in RPMI 1640 medium. Test materials were mixed according to the manufacturer's instructions and placed in contact with osteoblast cells. After the first, third and seventh days discs of materials with grown cells were fixed and examined by scanning electron microscopy. Results showed that after 7 days most of the osteoblasts were attached to the surface of both gray and white MTA and PC and appeared flat or round, however cells adjacent to IRM were round without any adhesion and spread. Based on the results of this study, human osteoblasts have a favorable response to gray and white MTA and Portland cement compared to IRM.