The Effect of Aluminum Injection in Lateral Ventricle on Sex Hormones in Male Rat.

Aluminum is an important voltage sensitive calcium channels blocker and enter the body from different sources. This ion interfere with biological function of calcium ion. Because GnRH synthesis and secretion from brain nucleus dependent on calcium ion, this experiment was performed to study the effect of aluminum on male rat''s sex hormones. The experiment was performed on foure groups of male rats, that the lateral ventricle were cannulated by sterotaxic surgery. Test group received 5.5 μmol ACSF containing 4.125 Рmol Aluminum in lateral ventricle for 20 days. Two series of these animals after cannulation received the same volume of ACSF with pH=7.2 and 3.4. The shame control animals did not received any agent after cannulation. At the end of experiment, animals were anesthetised with nesdonal (sodium thiopental) over dose and sacrified and blood samples were collected the vas deferense, epididymis and testis were removed and weighted. Epididymis and vas deferense were dissected, cut and diluted with normal saline. Spermatozoid were counted by hemocytometer and count were justified per gram of tissues. The sex hormones were measured by RI method. Statistic test was student t-test and the results are expressed as mean ± SE and P <0.05 were significant. Results show that sex hormone and spermatozoid concentration per gram of tissues in vas deferense, epididymis and testis weight in the group which received Aluminum in lateral ventricle decreased significantly compared with shame control. These result indicated that Aluminum injection in rat''s lateral ventricle can affect on sex hormones and Spermatozoid concentration per gram of these tissues and the weight of these organs, testis and the weight of these animals. Further studies will probably show the exact mechanism of Aluminum ion on sex hormone.