Extraction, Separation and Identification of Physalins from Physalis alkekengy Grown in Azarbayjan

In the present work, various extraction procedures including extraction with water and subsequently with chloroform, continuous extraction with chloroform, methanol and acetone were tested for the extraction of physalins from Physalis alkekengy grown in Eastesn Azarbayjan (Kaleibar). It was found that continuous extraction with acetone gives the best results in terms of amount of crude extract. After the preparative extraction, primary separation was carried out by column chromatography using silica gel as stationary phase and ethyl acetate: benzene (7:3) as solvent. In the second step, after choosing suitable solvent systems, the mixtures of physalins from previous step were separated by column and thin layer chromatography. Finally, physalins F and D together with a new compound were identified by using several spectroscopic techniques such as NMR, FT-IR and UV spectroscopy. An appropriate chemical structure based on the spectroscopic data proposed for the new compound.