THE EFFECT OF EGG DERIVED SPECIFIC ANTIBODY AND PROBIOTIC ON PREVENTION OF SALMONELLA ENTERITIDIS INFECTION IN BROILER CHICKENS

To evaluate the effect of probiotic (Primalac) and Salmonella enteritidis-specific IgY on prevention of Salmonella enteritidis infection in broiler chickens, four 33 week-old Single Comb White Leghorn hens were hyperimmunized with Salmonella enteritidis (SE) whole cell antigens obtained by ultrasonication and administrated at a protein concentration of 500 μg/ml after centrifugation. Primary immunization was performed with 250μg of the antigen prepared in equal volume of Freunds complete adjuvant and saline. Booster injections were done each 14 days for twice,using incomplete Freunds adjuvant. Bleedings were performed 20 days after first injection and eggs were collected. The presence of anti-Salmonella antibody IgY and IgG in egg yolk and serum respectively, was monitored by ELISA, during the immunization period. Body weight, feed intake and feed conversation were determined. Then two hundred forty male “Ross “day-old chicks were randomly assigned to 8 groups and 3 replications of 10 birds were grown for 56 days of experiment. Eight experimental groups identified with, S, P, A, SP, SA, AP, SPA, C. Four birds from four challenged groups(S), were orally inoculated with 0.5 Ml of S. enteritids that contained 1×106 cfu / ml on day 7.The groups that supplemented with antibody (A), received 15 ml of yolk contained antibody(1.5 ml/bird/day), from day 1 to end of the experiment. The probiotic treated groups(P) were received probiotic, 0.1% of feed and 0.5% of feed, during 1-21 and 22-56 days of experimental period respectively. One group as control© did not receive any treatment of probiotic and antibody.